• MGMT兔單克隆抗體

    MGMT兔單克隆抗體

    • 價(jià)格￥1400
    • 規格100ul
    • 貨號HKZ150363
    • 單位支
    • 品牌HaoKebio

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    HaoKebioHKZ150363MGMT兔單克隆抗體100ul支1400
    MGMT
    Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
    Catalog# ?BX50240?Clone# ?BP6217
    Predicted?Molecular Wt:
    22kDa
    Purity: ??ProA?affinity?purified?IgG
    Species Cross-reactivity:
    Human
    Form: ??Liquid
    Applications:
    IHC-P
    Swissprot?ID: ??P16455
    Background:
    Involved?in the cellular defense against the?biological?effects?of?O6-methylguanine?(O6-MeG)?in?DNA.?Repairs?alkylated?guanine?in?DNA?by stoichiometrically transferring the alkyl group at?the?O-6?position?to?acysteine?residue?in?the?enzyme.?This?is?a
    suicide?reaction: the enzyme?is?irreversibly?inactivated.
    Subcellular?location:
    Nucleus
    Recommended?method:
    Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
    Immunogen:
    A synthetic?peptide corresponding to?residues on the
    N-terminus?of?human?MGMT?was?used?as?an immunogen.
    Storage?Buffer:
    PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
    Storage conditions:
    -25°C?to?-18°C
    Storage instructions:
    Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
    Recommended?Dilutions:
    IHC-P:?????1:100-1:200
    Background?References:
    1. ?Bobola,?M.S.?et?al.?(2005) Clin.?Cancer?Res.?11,?2747-2755.
    2. ?Pollack,?I.F. et?al.?(2006) J. Clin.?Oncol.?24,?3431-3437.
    Immunohistochemistry
    (Formalin/PFA-fixed?paraffin-
    embedded?sections)?analysis of?????human endometrial?cancer tissue?labelling?MGMT?with?BP6217.
    Heat?mediated?antigen?retrieval?was?performed?using Tris/EDTA??buffer?pH?9.0

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