• Cytokeratin Pan兔單克隆抗體

    Cytokeratin Pan兔單克隆抗體

    • 價(jià)格¥600
    • 規格100ul
    • 貨號HKZ150266
    • 單位
    • 品牌HaoKebio

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    HaoKebioHKZ150266Cytokeratin Pan兔單克隆抗體100ul600
    Cytokeratin?Pan
    Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
    Catalog# ?BX50143 Clone#??BP6051&BP6058
    Predicted?Molecular Wt:
    45-66kDa
    Purity: ??ProA?affinity?purified?IgG
    Species Cross-reactivity:
    Human
    Form: ??Liquid
    Applications:
    IHC-P
    Swissprot?ID: ??P13645/P13647
    Background:
    The?keratins are the typical?intermediate filament?proteins of epithelia, showing?an?outstanding?degree?of?molecular?diversity.?Heteropolymeric filaments are formed?by?pairing of type?I and?type?II?molecules.?In?humans?54?functional?keratin?genes?exist.
    They are expressed?in?highly specific?patterns?related to the?epithelial type?and?stage?of?cellular?differentiation.?This cocktail antibody can detect?both type?Icytokeratins?and??type?II?cytokeratin.
    Subcellular?location:
    Cytoplasm
    Recommended?method:
    Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
    Immunogen:
    Synthetic?peptide corresponding to Type?ICytokeratins?residues?within?aa100-200 of?Type?ICytokeratins?was ????used as?an?immunogen.
    Synthetic?peptide corresponding to Type?II
    Cytokeratins?residues?within?aa300-400 of?Type?II Cytokeratins was?used as an?immunogen.
    Storage?Buffer:
    PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
    Storage conditions:
    -25°C?to?-18°C
    Storage instructions:
    Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
    Recommended?Dilutions:
    IHC-P:?????1:100-1:200
    Background?References:
    1.?Spagnolo?DV,?et?al. Am?J?Clin?Pathol.?1985?Dec;84(6):697-704.
    2. ?Eichner?R, et?al. J?Cell?Biol.?1984 Apr;98(4):1388-96.
    Immunohistochemistry
    (Formalin/PFA-fixed
    paraffin-embedded
    sections)?analysis of
    esophagus tissue?labelling?Cytokeratin?Pan with
    BP6051&BP6058.?Heat mediated?antigen
    retrieval?was?performed????using Tris/EDTA?buffer?pH?9.0?

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