• MSH2鼠單克隆抗體

    MSH2鼠單克隆抗體

    • 價(jià)格￥1000
    • 規格100ul
    • 貨號HKZ150265
    • 單位支
    • 品牌HaoKebio

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    HaoKebioHKZ150265MSH2鼠單克隆抗體100ul支1000
    MSH2
    Mouse?Monoclonal Antibody?Product?Datasheet
    Catalog# ?BX50142???Clone# ?BPM6143
    Predicted?Molecular Wt:
    105kDa
    Purity: ??ProG?affinity?purified?IgG
    Species Cross-reactivity:
    Human
    Form: ??Liquid
    Applications:
    IHC-P
    Swissprot?ID: ??P43246
    Background:
    MSH2?is?a?105?kDa?nuclear?antigen?and?encodes?a?protein?of?934?amino?acids. The?MSH2?antibody?gene?is?one?of?only?4?known?to encode?proteins?involved?in the?repair of?mismatch?nucleotides following?DNA?replication or?repair.?Mutations?in the?MSH2 gene??contribute to the development of sporadic colorectal?carcinoma.?MSHS?mutations?are?responsible?for?50%?of?hereditary?non-
    polyposis colorectal cancer?(HNPCC). The?repair of?mismatch?DNA?is essential to?maintaining?the?integrity?of?genetic?information?overtime. An alteration of?microsatellite?repeats?is the?result of slippage?owing?to?strand?misalignment?during?DNA?replication????and?is?referred to as?microsatellite?instability?(MSI). These defects?in?DNA?repair?pathways?have?been?related to?human
    carcinogenesis.?MSH-2?is?involved?in the?initial cognition of?mismatch?nucleotides during the?replication?mismatch?repair?process.??It?is thought that after?MSH2?binds to a?mismatched?DNA duplex?it?is joined?by?a?heterodimer?of?MLH1?and?PMSH,?which?together?help facilitate the?later steps?in?mismatch?repair.
    Subcellular?location:
    Nucleus
    Recommended?method:
    Heat?induced epitope?retrieval with Tris-EDTA?buffer
    (pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)
    for?30?minutes.
    Immunogen:
    Recombinant?fragment?(around?aa?327-427) of?human
    MSH2?protein
    Storage?Buffer:
    PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA
    0.05%.
    Storage conditions:
    -25°C?to?-18°C
    Storage instructions:
    Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store
    at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
    Recommended?Dilutions:
    IHC-P:?????1:100-1:200
    Background?References:
    1.?Thibodeau?SN,?French?AJ, et?al.Cancer?Res?1996?Nov
    1;56(21):4836-40
    2. ?National Committee for Clinical?Laboratory Standards?(NCCLS). Villanova,?PA?1991;7(9). Order?code?M29-P.
    Immunohistochemistry
    (Formalin/PFA-fixed
    paraffin-embedded
    sections)?analysis of
    human colon?carcinoma
    tissue from?Lynch disease
    labelling?MSH2 with
    BPM6143.?Heat?mediated antigen?retrieval?was
    performed?using Tris/EDTA
    buffer?pH?9.0

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