• S100兔單克隆抗體

    S100兔單克隆抗體

    • 價(jià)格￥600
    • 規格100ul
    • 貨號HKZ150206
    • 單位支
    • 品牌HaoKebio

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    HaoKebioHKZ150206S100兔單克隆抗體100ul支600
    S100
    Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
    Catalog# ?BX50083?Clone# ?BP6088
    Predicted?Molecular Wt:
    21kDa
    Purity: ??ProA?affinity?purified?IgG
    Species Cross-reactivity:
    Human
    Form: ??Liquid
    Applications:
    IHC-P
    Swissprot?ID: ??P04271
    Background:
    S100?is a?21kDa?highly?acidic and water?soluble?protein, which?is?expressed?in?a?wide?variety?of?normal?and?neoplastic?cells?of?mesodermal,?neuroectodermal, and epithelial origin. The?protein?is a?dimer,?having?two?subunits,?alpha?and?beta,?with
    extensive?sequence?homology. There?are?3 forms?of?S-100?protein:?alpha-alpha,?known?as?S-100A0;?alpha-beta,?known?as?S-
    100A?and?beta-beta,?known?as?S-100B. The?immunohistochemical?evaluation?of?S-100?(beta)?protein?expression?is?important?in the diagnosis of?undifferentiated?malignant tumours of?unknown?primary origin?and?should?be?included?in?the?so-called
    primary?panel. S100 is?a?very?sensitive?marker?for?malignant?melanoma?of?all?types.?S100?may?be?used?in?the?differential diagnosis of sarcomas and spindle?cell?tumours.Subcellular?location:
    Cytoplasm,?Nuclears
    Recommended?method:
    Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
    Immunogen:
    Synthetic?peptide corresponding to?residues within?aa50-150 of?S100 was?used?as?an?immunogen.
    Storage?Buffer:
    PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
    Storage conditions:
    -25°C?to?-18°C
    Storage instructions:
    Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
    Recommended?Dilutions:
    IHC-P:?????1:100-1:200
    Background?References:
    1. ?Hagen?EC, Vennegoor?C, Schlingemann?RO, Van?der Velde?EA,?Ruiter?DJ.?Histopathol?1986;10:689-700.
    2.?Schafer?BW,?Heizmann?CW. Trends?Biochem?Sci.?1996?Apr;21(4):134-40.
    Immunohistochemistry?(Formalin/PFA-fixed
    paraffin-embedded
    sections)?analysis of
    melanoma?labelling?S100?with?BP6088.?Heat
    mediated?antigen
    retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0.

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