• Villin兔單克隆抗體

    Villin兔單克隆抗體

    • 價(jià)格￥600
    • 規格100ul
    • 貨號HKZ150184
    • 單位支
    • 品牌HaoKebio

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    HaoKebioHKZ150184Villin兔單克隆抗體100ul支600
    Villin
    Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
    Catalog# ?BX50061?Clone# ?BP6066
    Predicted?Molecular Wt:
    93kDa
    Purity: ??ProA?affinity?purified?IgG
    Species Cross-reactivity:
    Human
    Form: ??Liquid
    Applications:
    IHC-P
    Swissprot?ID: ??P09327
    Background:
    Villinisa 93?kDa calcium-regulated, actin-binding?protein that?plays a?role?in?regulating?actin?filament?assembly.?It?is?a?major?constituent?in the?microvilli, which compose the?brush?border of epithelial?cells forming?absorptive?surfaces?of the?intestinal?and?renal?proximal tubular epithelia.
    In?normal?human tissue, villin?is expressed?by a?limited?number of?simple?epithelia?of the?gastrointestinal?and?urogenital?tract.?In tumor tissue,?it?is always expressed?in colorectal?cancer,?hepatocellular?carcinoma,?endometrium?cancer,?as well?as?cancers??of ovary?and?lung.
    Villinisa?useful aid for classification of?primary and?metastatic?colorectal?carcinomas.
    Subcellular?location:
    Cytoplasm
    Recommended?method:
    Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
    Immunogen:
    Synthetic?peptide corresponding to Villin?residues
    within?aa600-700 of??Villin?was?used?as?an?immunogen.
    Storage?Buffer:
    PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
    Storage conditions:
    -25°C?to?-18°C
    Storage instructions:
    Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
    Recommended?Dilutions:
    IHC-P:?????1:100-1:200
    Background?References:
    1. ?R?Mollet?al.?Histochem?Cell?Biol?(2008)?129:705–733.
    2. ?R?Mollet?al.?Cell, Vol.?31,?1?l-24,?November?1982.
    Immunohistochemistry?(Formalin/PFA-fixedparaffin-embedded
    sections?analysis of?colon?tissue?labelling Villin with?BP6066.?Heat?mediated ???antigen?retrieval was
    performed?using
    Tris/EDTA?buffer?pH?9.0

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