• WT1兔單克隆抗體

    WT1兔單克隆抗體

    • 價(jià)格￥1000
    • 規格100ul
    • 貨號HKZ150180
    • 單位支
    • 品牌HaoKebio

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    HaoKebioHKZ150180WT1兔單克隆抗體100ul支1000
    WT1
    Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
    Catalog# BX50057?Clone# ?BP6062
    Predicted?Molecular Wt:
    49kDa
    Purity: ??ProA?affinity?purified?IgG
    Species Cross-reactivity:
    Human
    Form: ??Liquid
    Applications:
    IHC-P
    Swissprot?ID: ??P19544
    Background:
    The WT1 gene?located at chromosome?11p13 codes for?a?transcription?factor,a?DNA-binding?nucleoprotein,?that?plays?a?role?primarily?in the development of genitourinary organs. There?are?at?least?eight?isoforms?ranging?between?52?and?62?kDa
    produced?by a combination of alternative?splicing?and?RNA?editing.?WT1?is?synthesized?and?reside?in?the?cytoplasm?in?an
    inactive form. When activated through?phosphorylation?it?is translocated to the?nucleus. WT1?influences?cell?proliferation?by?suppressing?bcl-2 and?regulating?cadherin?and?p53.
    In?normal epithelia,?nuclear WT1 expression?is?largely?restricted to?ovary?(surface?epithelium?and?inclusion?cysts)?and?fallopian?tube, while WT1?is?not found?in endometrial or?cervical?epithelium. As?regards?nonepithelial?cells,?nuclear?WT1?is?found?in
    mesothelium and some submesothelial stromal?cells, stromal?cells?of the?female?genital?tract,?testicular?non-germinal?cells,????and?kidney?(podocytes).?In tumor tissues, WT1?is detected?in tumor cells?of?Wilms’ Tumor?(also??known?as?nephroblastoma)????and?mesothelioma. Additionally, WT1 expression?has?been found?in ovarian?serous?carcinomas?and?some?breast?carcinomas.
    WT1?is?particularly?used for distinguishing?malignant?mesothelioma and ovarian?serous?carcinoma from?nonserous?carcinomas. As for?malignant?mesothelioma, calretinin and WT1 are?superior?to?cytokeratin?5/6,?N-cadherin?and
    thrombomodulin. WT1?is also applicable for the differential?diagnostic?of?small?cell?childhood?tumours.
    Subcellular?location:
    Nucleus
    Recommended?method:
    Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
    Immunogen:
    Synthetic?peptide corresponding to WT1?residues
    within?aa1-100 of??WT1 was?used?as?an?immunogen.
    Storage?Buffer:
    PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
    Storage conditions:
    -25°C?to?-18°C
    Storage instructions:
    Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
    Recommended?Dilutions:
    IHC-P:?????1:100-1:200
    Background?References:
    1.?Carpentieri?DF?etal.?Mod?Pathol.?2002?Oct;15(10):1080-6.
    2.?Goldstein?NS?etal. Am?J?Clin?Pathol.?2002?Apr;117(4):541-5.
    Immunohistochemistry?(Formalin/PFA-fixed
    paraffin-embedded
    sections)?analysis of
    ovarian cancer?labelling?WT1 with?BP6062.?Heat mediated?antigen
    retrieval was?performed?using Tris/EDTA?buffer????pH?9.0.

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