• Ki-67兔單克隆抗體

    Ki-67兔單克隆抗體

    • 價(jià)格￥600
    • 規格100ul
    • 貨號HKZ150163
    • 單位支
    • 品牌HaoKebio

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    HaoKebioHKZ150163Ki-67兔單克隆抗體100ul支600
    Ki-67
    Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
    Catalog# ?BX50040?Clone# ?BP6045
    Predicted?Molecular Wt:
    359kDa
    Purity: ??ProA?affinity?purified?IgG
    Species Cross-reactivity:
    Human
    Form: ??Liquid
    Applications:
    IHC-P
    Swissprot?ID: ??P46013
    Background:
    Antigen?Ki-67?is a?nuclear?protein that?is associated with and?may?be?necessary?for?cellular?proliferation.?Furthermore,?it?is?associated with?ribosomal?RNA transcription.
    Ki-67 was?present?in the?nuclei of cells?in the G1,S,and?G2?phases?of the?cell?division?cycle?as?well?as?in?mitosis.?Quiescent?or?resting?cells?in?the?G0?phase?did?not?express?the?Ki-67?antigen.
    Ki-67?protein was?used as diagnostic tools?in?different types?of?neoplasms?because?it was?present?in?all?proliferating?cells
    (normal and tumor cells) and was?an?excellent?operational?marker?to?determine?the?growth?fraction?of?a?given?cell?population.
    Subcellular?location:
    Nucleus
    Recommended?method:
    Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
    Immunogen:
    Synthetic?peptide corresponding to?Ki-67?residues?within?aa1000-1100 of?Ki-67 was?used?as?an
    immunogen.
    Storage?Buffer:
    PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
    Storage conditions:
    -25°C?to?-18°C
    Storage instructions:
    Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
    Recommended?Dilutions:
    IHC-P:?????1:100-1:200
    Background?References:
    1.?Scholzen?T, et.al, J?Cell?Physiol.?2000?Mar;182(3):311-?22.
    2. ?Inwald?EC, et.al,?Breast?Cancer?Res?Treat.?2013?Jun;139(2):539-52.
    Immunohistochemistry?(Formalin/PFA-fixed
    paraffin-embedded
    sections analysis?of
    tonsil tissue?labelling??Ki-?67 with?BP6045.?Heat
    mediated?antigen
    retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0

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