Cytokeratin 20兔單克隆抗體

價(jià)格￥600
規格100ul
貨號HKZ150148
單位支
品牌HaoKebio

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產(chǎn)品參數

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品牌	商品編碼	商品名稱(chēng)	規格	單位	單價(jià)
HaoKebio	HKZ150148	Cytokeratin 20兔單克隆抗體	100ul	支	600

Cytokeratin?20
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# BX50025?Clone# ?BP6030
Predicted?Molecular Wt:
48kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P35900
Background:
Cytokeratin?20?is the simple-epithelial?keratin with the?most?restricted expression?pattern.?For?pair formation,?its type?II?partner?usually appears to?be cytokeratin?8.
The?remarkable expression spectrum of cytokeratin?20 among?normal?tissues?comprises?gastric?foveolar?epithelium?and?small?and?large?intestinal epithelium and,?in addition, the?urothelium?and?certain?neuroendocrine?cells,?in?particular?Merkel?cells of?the skin.?In tumor tissues, cytokeratin?20?is expressed?in?adenocarcinomas?of the?colon,?stomach,?pancreas,?bile system?and?????mucinous ovarian tumors, transitional cell carcinomas?of the?urinary tract,?and?Merkel?cell?carcinomas.
Cytokeratin?20?is often?used?in conjunction with CK7?and?other?antibodies?in?distinguishing?colon?carcinomas?(CK20+) from?ovarian,?pulmonary, and?breast carcinomas.
?Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to cytokeratin?20
residues?within?aa324‐424 of?cytokeratin?20?was?used as an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1.?Scott?MP, et.al, Am?J?Dermatopathol.?1999?Feb;21(1):16-20.
2. ?Moll?R, et.al, Am?J?Pathol.?1992?Feb;140(2):427-47.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded?sections?analysis of?human?colonic
adenocarcinoma tissue
labelling Cytokeratin?20?with?BP6030.?Heat?mediated
antigen?retrieval?was
performed?using Tris/EDTA?buffer?pH?9.0

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