• Lambda Light Chains兔單克隆抗體

    Lambda Light Chains兔單克隆抗體

    • 價(jià)格￥600
    • 規格100ul
    • 貨號HKZ150252
    • 單位支
    • 品牌HaoKebio

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    HaoKebioHKZ150252Lambda Light Chains兔單克隆抗體100ul支600
    Lambda?Light Chains
    Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
    Catalog# ?BX50129?Clone# ?BP6133
    Predicted?Molecular Wt:
    23kDa
    Purity: ??ProA?affinity?purified?IgG
    Species Cross-reactivity:
    Human
    Form: ??Liquid
    Applications:
    IHC-P
    Swissprot?ID: ??P0CG04
    Background:
    Lambda?light chains are?polypeptide chains?located?in?the cell?membrane?and?cytoplasmic?regions?of?normal?B?cells?and
    plasma cells. The combination of?lambda?light chains?and?heavy?chains?forms?immunoglobulin?molecules.?There?are?two
    classes of?light chains found?in?immunoglobulins,?kappa?light chains and?lambda?light?chains.?Light?chain?production?by
    lymphoid cells?is genetically?restricted such that the?immunoglobulin?molecules?produced?by?an?individual?cell will?only
    contain a single?light chain class.?The?normal?human?kappa/lambda?ratio?is?approximately?2:1.?The?presence?of?clear?cut?light?chain?restriction with a?kappa/lambda?ratio?more than?10:1?is consistent with?a?malignant?proliferation.
    This antibody?recognizes?lambda?light chains of?human?immunoglobulins, which?may?be?useful?in the?identification?of
    leukemias,?plasmacytomas, and certain?non-Hodgkin’s?lymphomas. The?most common feature?of these?malignancies?is the?restricted expression of a?single?light?chain?class.
    Subcellular?location:
    Cytoplasm
    Recommended?method:
    Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
    Immunogen:
    Synthetic?peptide corresponding to?residues within?N-?terminus of?Immunoglobulin?lambda constant?1 was????used as?an?immunogen.
    Storage?Buffer:
    PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
    Storage conditions:
    -25°C?to?-18°C
    Storage instructions:
    Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
    Recommended?Dilutions:
    IHC-P:?????1:100-1:200
    Background?References:
    1. ?Kremer?M,et?al. Virchows?Arch.?2005?Dec; 447(6):920-?37.
    2. ?Marshall-Taylor?CE, et?al. Appl?Immunohistochem?Mol Morphol.?2002 Sep;?10(3):258-62.
    Immunohistochemistry
    (Formalin/PFA-fixed
    paraffin-embedded
    sections)?analysis of tonsil?tissue?labelling?Lambda
    Light?Chains??with?BP6133.?Heat?mediated?antigen
    retrieval was?performed????using Tris/EDTA?buffer?pH?9.0?

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