杭州浩克生物
p16鼠單克隆抗體
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| 品牌 | 商品編碼 | 商品名稱(chēng) | 規格 | 單位 | 單價(jià) |
|---|---|---|---|---|---|
| HaoKebio | HKZ150139 | Cytokeratin 5兔單克隆抗體 | 100ul | 支 | 600 |
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
?
Catalog# ?BX50016?Clone# ?BP6021
?
Predicted?Molecular Wt:
62kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P13647
?
?
Background:
Cytokeratin?5?is a?basic?protein that?is closely?related to?cytokeratin?6. They share similar tissue distribution?and
are found?in various?proportions?in?many?non-
keratinizing stratified squamous epithelia?eg. tongue??mucosa, as well as?in?basal?epithelia?of trachea,?basal?cells of epidermis,?hair follicles, sebaceous?and?sweat?glands of skin,?luminal cells of the?mammary?gland,?????basal cells of?prostate,?urothelium, vagina?and
endocervical?mucosa. Cytokeratins 5 and?6?are?also?expressed?in?basal cell epitheliomas, squamous?cell
carcinomas of skin, tongue, epiglottis?and?of the?rectal-?anal?region.?Point?mutations?in the cytokeratin 5 gene
at?locus?12q11-q13 can?cause?various?types?of epidermolysis?bullosa simplex.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
human skin tissue?labelling?cytokeratin?5 with?BP6021.?Heat?mediated?antigen
retrieval?was?performed????using Tris/EDTA?buffer?pH?9.0
Immunogen:
Synthetic?peptide?according?to?the?aa490-590?(C-
terminal) of ?cytokeratin 5 was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Mukhopadhyay?S., et.al.??Am?J?Surg Pathol.?2011 Jan; 35(1):15-25.
2. ??KargiA, Gurel?D., et.al. Appl?Immunohistochem?Mol Morphol.?2007?Dec;?15(4):415-20.
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