• TFE3兔單克隆抗體

    TFE3兔單克隆抗體

    • 價(jià)格￥600
    • 規格100ul
    • 貨號HKZ150326
    • 單位支
    • 品牌HaoKebio

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    HaoKebioHKZ150326TFE3兔單克隆抗體100ul支600
    TFE3
    Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
    Catalog# ?BX50203?Clone# ?BP6180
    Predicted?Molecular Wt:
    62kDa
    Purity: ??ProA?affinity?purified?IgG
    Species Cross-reactivity:
    Human
    Form: ??Liquid
    Applications:
    IHC-P
    Swissprot?ID: ??P19532
    Background:
    Transcription factor that acts as a?master?regulator?of?lysosomal?biogenesis?and?immune?response.?Specifically?recognizes?and?binds?E-box sequences; efficient?DNA-binding?requires dimerization with?itself or?with?another?MiT/TFE?family?member?such
    as TFEB or?MITF.?Involved?in the cellular?response to amino?acid?availability?by?acting?downstream?of?MTOR:?in?the?presence?of?nutrients, TFE3?phosphorylation?by?MTOR?promotes?its cytosolic?retention and subsequent?inactivation.?Upon starvation?or
    lysosomal stress,?inhibition of?MTOR?induces TFE3 dephosphorylation,?resulting?in?nuclear?localization and?transcription?factor?activity?.?In association with TFEB, activates the expression?of?CD40L?in?T-cells,?thereby?playing?a?role?in?T-cell-dependent
    antibody?responses?in activated CD4+ T-cells and thymus-dependent?humoral?immunity?. Specifically?recognizes the?MUE3
    box,a subset of?E-boxes,?present?in the?immunoglobulin enhancer.?It?also?binds very?well to?a?USF/MLTF?site.?Acts?as?a?positive?regulator of?browning of adipose tissue?by?promoting expression?of target?genes;?mTOR-dependent?phosphorylation
    promotes cytoplasmic?retention of TFE3 and?inhibits?browning of adipose?tissue.?Maintains?the?pluripotent?state?of?embryonic?stem cells?by?promoting the expression of genes such?as?ESRRB;?mTOR-dependent?nuclear?exclusion?promotes?exit?from
    pluripotency?.?Required to?maintain the?naive?pluripotent state of?hematopoietic stem?cell;?mTOR-dependent?cytoplasmic?retention of TFE3?promotes the exit of?hematopoietic stem?cell from?pluripotency.
    Subcellular?location:
    Nucleus
    Recommended?method:
    Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
    Immunogen:
    Synthetic?peptide was?used as an?immunogen.
    Storage?Buffer:
    PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
    Storage conditions:
    -25°C?to?-18°C
    Storage instructions:
    Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
    Recommended?Dilutions:
    IHC-P:?????1:100-1:200
    Background?References:
    1. ?Miller, A.J. et?al.?(2005) J?Biol?Chem?280,?146-55
    2. ?Kauffman,?E.C. et?al.?(2014)?Nat?Rev?Urol?11, 465-75.
    Immunohistochemistry
    (Formalin/PFA-fixed
    paraffin-embedded
    sections)?analysis of?Renal?cell?carcinoma?labelling
    TFE3 with?BP6180.?Heat
    mediated?antigen?retrieval?was?performed?using
    Tris/EDTA?buffer?pH?9.0.

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