Cytokeratin (HMW)兔單克隆抗體Cocktail

價(jià)格￥600
規格100ul
貨號HKZ150234
單位支
品牌HaoKebio

產(chǎn)品介紹

產(chǎn)品參數

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品牌	商品編碼	商品名稱(chēng)	規格	單位	單價(jià)
HaoKebio	HKZ150234	Cytokeratin (HMW)兔單克隆抗體Cocktail	100ul	支	600

Cytokeratin?(HMW)
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50111 Clone# ?BP6021/BP6077
Purity:
ProA affinity?purified?IgG
Species Cross-reactivity:
Human
Form:
Liquid
Applications:
IHC-P
Background:
In?normal tissues, cytokeratin?(high?molecular weight)?is?mainly expressed?in?normal squamous?epithelium,?ductal?epithelium?and other?laminated epithelium.?Positive expression was found?in?lungsquamous?cell?carcinoma,?breast?cancer,?pancreatic
cancer, cholangiocarcinoma and?bladder transitional cell carcinoma.
Anti-cytokeratin,?high?molecular weight ?is capable of?recognizing?all?basic?keratins;?therefore,?it?is?a?broadly?reactive?antibody?staining?most epithelia and their?neoplasms.?Members of the?acidic?and?basic?subfamilies?are found together?in?pairs.?Since
each epithelium contains at?least one?acidic?and?one?basic?keratin, this?antibody?is?used?to?observe?the?distribution?of?keratin-?containing cells?in?normal epithelia and to?identify?neoplasms derived?from?such?epithelium.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?residues within
aa200-300 of??Cytokeratin?1 was?used?as?an immunogen.
Synthetic?peptide?according?to?the?aa490-590?(C-
terminal) of ?Cytokeratin 5?was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?Kriho?UK, et?al. Virehows?Arch.?1997;?431:139-47.
2. ?D?Oh,?RA?Prayson. Arch?Pathol?Lab?Med—Vol?123,?October?1999:917-920.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
esophagus ?carcinoma
tissue?labelling?Cytokeratin?(HMW)?.?Heat?mediated
antigen?retrieval?was
performed?using Tris/EDTA?buffer?pH?9.0?

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