• Phospho histone H3( PHH3)兔單克隆抗體

    Phospho histone H3( PHH3)兔單克隆抗體

    • 價(jià)格￥1000
    • 規格100ul
    • 貨號HKZ150210
    • 單位支
    • 品牌HaoKebio

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    HaoKebioHKZ150210Phospho histone H3( PHH3)兔單克隆抗體100ul支1000
    Phosphohistone?H3 (PHH3)
    Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
    Catalog# ?BX50087?Clone# ?BP6092
    Predicted?Molecular Wt:
    15kDa
    Purity: ??ProA?affinity?purified?IgG
    Species Cross-reactivity:
    Human
    Form: ??Liquid
    Applications:
    IHC-P
    Swissprot?ID: ??P68431
    Background:
    Phosphohistone?H3?(PHH3)?is?a?marker?specific?for?cells?undergoing?mitosis. Serine?10?of?Histone?H3?is?phosphorylated?in association with?mitotic chromatin condensation?in?late G2?and?M?phase?of the?cell?cycle?and thus,?PHH3?can?distinguish??mitosis from apoptotic?nuclei.
    The?range?of?percentage?PHH3?positive?tumor?nuclei?was?from?0.0 to?6.6%?(median?value?0.8%).?Increased?expression?of?PHH3?was?significantly?associated?with?tumor?thickness?(p?= 0.031),?presence?of?tumor?ulceration?(p?=0.041) and?tumor?necrosis?(p?=?0.027),?but?not?with?Clark’s?level?of?invasion.?High?levels?of?PHH3 was?associated?with?increased?mitotic?count?(p?=?0.003)?and ??high?Ki-67 expression?(p =?0.002).
    For central?nervous system tumors,?melanoma, soft tissue tumors,?GIST,?etc.,?PHH3?mAb?is?helpful for?tumor?pathological?classification and?prognosis.
    Subcellular?location:
    Nucleus
    Recommended?method:
    Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
    Immunogen:
    Synthetic?peptide corresponding to?Phosphohistone?H3?(PHH3)?residues?within?aa1-100?(phosphoS10) ?was
    used as?an?immunogen.
    ?Storage?Buffer:
    PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
    Storage conditions:
    -25°C?to?-18°C
    Storage instructions:
    Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
    Recommended?Dilutions:
    IHC-P:?????1:100-1:200
    Background?References:
    1.?TharejaS, et?al. Am?J?Dermatopathol. 2014 Jan;?36(1):64-7.
    2.?Casper?DJ, et?al. Am?J?Dermatopathol.?2010 Oct;?32(7):650-4.
    Immunohistochemistry?(Formalin/PFA-fixed
    paraffin-embedded
    sections analysis?of tonsil
    tissue?labelling
    Phosphohistone?H3
    (PHH3) with?BP6092.
    Heat?mediated?antigen???retrieval?was?performed?using Tris/EDTA?buffer????pH?9.0

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